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Registro Completo |
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Biblioteca(s): |
Embrapa Pesca e Aquicultura. |
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Data corrente: |
28/04/2021 |
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Data da última atualização: |
28/04/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CORNACINI, M. R.; MANOEL, R. O.; ALCANTARA, M. A. M.; MORAES, M. L. T.; SILVA, E. A. A.; PEREIRA NETO, L. G.; SEBBENN, A. M.; ROSSINI, B. C.; MARINO, C. L. |
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Afiliação: |
MAIARA R. CORNACINI, UNESP, Botucatu-SP; RICARDO O. MANOEL, UNESP, Botucatu-SP; MARCELO A. M. ALCANTARA, UNESP, Botucatu-SP; MARIO L. T. MORAES, UNESP, Ilha Solteira-SP; EDVALDO A. A. SILVA, UNESP, Botucatu-SP; LEONEL GONCALVES PEREIRA NETO, CNPASA; ALEXANDRE M. SEBBENN, INSTITUTO FLORESTAL DE SÃO PAULO, Piracicaba-SP; BRUNO C. ROSSINI, UNESP, Botucatu-SP; CELSO L. MARINO, UNESP, Botucatu-SP. |
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Título: |
Detection and application of novel SSR markers from transcriptome data for Astronium fraxinifolium Schott, a threatened Brazilian tree species. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Molecular Biology Reports, 2021. |
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ISSN: |
0301-4851 |
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DOI: |
https://doi.org/10.1007/s11033-021-06338-5 |
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Idioma: |
Inglês |
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Conteúdo: |
Astronium fraxinifolium is an endangered tree species from Brazil. Due to its significance in environmental reforestation, as well as the continued exploitation of its wood, it is necessary to develop management programs that support the conservation of the species. Simple sequence repeats (SSR) or microsatellite markers are widely used in population genetic studies across a range of diverse organisms. In this study, we present the first SSR markers developed for A. fraxinifolium as well as their frequency and distribution based on transcriptome data. From transcriptome data, we identified more than 100 thousand sequences presenting microsatellites, with a predominant distribution of trinucleotide repeats. From the initial screening, we selected 20 microsatellite loci which were validated and evaluated for genetic indices in two natural populations. All loci were polymorphic, ranging from four to 11 alleles per locus. The observed and expected heterozygosities ranged from 0 to 1.0 and from 0.533 to 1.0, respectively, while the genetic differentiation (GST = 0.363) was greater within than between populations. The developed SSR loci from RNA-Seq data provides a foundation for future studies on genetic diversity and population structure, mating system, and gene flow for A. fraxinifolium populations and related species, aiming at conservation and management. |
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Palavras-Chave: |
Conservation genetics; Microsatellite markers. |
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Thesagro: |
Anacardiaceae; Astronium Fraxinifolium. |
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Thesaurus Nal: |
Conservation plants; Genetic markers; Microsatellite repeats; Population genetics. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/222904/1/mbr-2021.pdf
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Marc: |
LEADER 02452naa a2200337 a 4500
001 2131540
005 2021-04-28
008 2021 bl uuuu u00u1 u #d
022 $a0301-4851
024 7 $ahttps://doi.org/10.1007/s11033-021-06338-5$2DOI
100 1 $aCORNACINI, M. R.
245 $aDetection and application of novel SSR markers from transcriptome data for Astronium fraxinifolium Schott, a threatened Brazilian tree species.$h[electronic resource]
260 $c2021
520 $aAstronium fraxinifolium is an endangered tree species from Brazil. Due to its significance in environmental reforestation, as well as the continued exploitation of its wood, it is necessary to develop management programs that support the conservation of the species. Simple sequence repeats (SSR) or microsatellite markers are widely used in population genetic studies across a range of diverse organisms. In this study, we present the first SSR markers developed for A. fraxinifolium as well as their frequency and distribution based on transcriptome data. From transcriptome data, we identified more than 100 thousand sequences presenting microsatellites, with a predominant distribution of trinucleotide repeats. From the initial screening, we selected 20 microsatellite loci which were validated and evaluated for genetic indices in two natural populations. All loci were polymorphic, ranging from four to 11 alleles per locus. The observed and expected heterozygosities ranged from 0 to 1.0 and from 0.533 to 1.0, respectively, while the genetic differentiation (GST = 0.363) was greater within than between populations. The developed SSR loci from RNA-Seq data provides a foundation for future studies on genetic diversity and population structure, mating system, and gene flow for A. fraxinifolium populations and related species, aiming at conservation and management.
650 $aConservation plants
650 $aGenetic markers
650 $aMicrosatellite repeats
650 $aPopulation genetics
650 $aAnacardiaceae
650 $aAstronium Fraxinifolium
653 $aConservation genetics
653 $aMicrosatellite markers
700 1 $aMANOEL, R. O.
700 1 $aALCANTARA, M. A. M.
700 1 $aMORAES, M. L. T.
700 1 $aSILVA, E. A. A.
700 1 $aPEREIRA NETO, L. G.
700 1 $aSEBBENN, A. M.
700 1 $aROSSINI, B. C.
700 1 $aMARINO, C. L.
773 $tMolecular Biology Reports, 2021.
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Registro original: |
Embrapa Pesca e Aquicultura (CNPASA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
06/12/2018 |
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Data da última atualização: |
06/12/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
ALLIEVI, M. J.; SILVEIRA, D. D.; CANTAO, M. E.; BELLI FILHO, P. |
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Afiliação: |
MARIA JOANA ALLIEVI, UFSC/Departamento de Engenharia Sanitária e Ambiental; UFSC/Departamento de Engenharia Sanitária e Ambiental; MAURICIO EGIDIO CANTAO, CNPSA; PAULO BELLI FILHO, UFSC/Departamento de Engenharia Sanitária e Ambiental. |
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Título: |
Bacterial community diversity in a full scale biofilter treating wastewater odor. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Water Science & Technology, v. 77, n. 8, 2018. |
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DOI: |
10.2166/wst.2018.114. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Constantly, the odors coming from sewage plants are considered a problem by the population. The purpose of this study was to evaluate the microbial community present in a full scale biofilter used for odor treatment. The filter was packed with peat. The main gas treated was hydrogen sulphide (H2S). The removal efficiency reached 99%, with an empty bed residence time of 30 seconds. Molecular analysis can enhance our understanding of the microbial communities in biofilters treating wastewater odor. The analysis made to characterize microbial community was High-throughput 16S rRNA sequencing analysis MiSeq® Illumina. The sampling, carried out in the year 2015, was seasonal (summer and winter) and spatial (depth and position in the biofilter). In this study, a total of 206,174 raw sequence reads for six samples were analyzed using Mothur software (v 1.33.3) based on MiSeq SOP protocol. After Mothur analysis, the results of the bacterial community were explored at the Phylum and Genus levels. In this study, the efficiency removal of hydrogen sulfide reached values greater than 99% during the monitoring, and the main bacterial genera found were Acidotermus, Telmatobacter, Methylovirgula and Bryobacter representing the bacterial community active in the transformation of H2S into a system with long operating time. |
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Palavras-Chave: |
Biofiltro; Gás sulfídrico; Sulphide gas. |
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Thesagro: |
Águas Residuais; Contaminação Bacteriana; Odor. |
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Thesaurus NAL: |
Bacterial communities; Biofilters; Odor control; Sulfides; Wastewater. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02180naa a2200301 a 4500
001 2100829
005 2018-12-06
008 2018 bl uuuu u00u1 u #d
024 7 $a10.2166/wst.2018.114.$2DOI
100 1 $aALLIEVI, M. J.
245 $aBacterial community diversity in a full scale biofilter treating wastewater odor.$h[electronic resource]
260 $c2018
520 $aAbstract: Constantly, the odors coming from sewage plants are considered a problem by the population. The purpose of this study was to evaluate the microbial community present in a full scale biofilter used for odor treatment. The filter was packed with peat. The main gas treated was hydrogen sulphide (H2S). The removal efficiency reached 99%, with an empty bed residence time of 30 seconds. Molecular analysis can enhance our understanding of the microbial communities in biofilters treating wastewater odor. The analysis made to characterize microbial community was High-throughput 16S rRNA sequencing analysis MiSeq® Illumina. The sampling, carried out in the year 2015, was seasonal (summer and winter) and spatial (depth and position in the biofilter). In this study, a total of 206,174 raw sequence reads for six samples were analyzed using Mothur software (v 1.33.3) based on MiSeq SOP protocol. After Mothur analysis, the results of the bacterial community were explored at the Phylum and Genus levels. In this study, the efficiency removal of hydrogen sulfide reached values greater than 99% during the monitoring, and the main bacterial genera found were Acidotermus, Telmatobacter, Methylovirgula and Bryobacter representing the bacterial community active in the transformation of H2S into a system with long operating time.
650 $aBacterial communities
650 $aBiofilters
650 $aOdor control
650 $aSulfides
650 $aWastewater
650 $aÁguas Residuais
650 $aContaminação Bacteriana
650 $aOdor
653 $aBiofiltro
653 $aGás sulfídrico
653 $aSulphide gas
700 1 $aSILVEIRA, D. D.
700 1 $aCANTAO, M. E.
700 1 $aBELLI FILHO, P.
773 $tWater Science & Technology$gv. 77, n. 8, 2018.
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Embrapa Suínos e Aves (CNPSA) |
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